I’m working on a biology question and need an explanation to help me study.
Create a table below with the color of the food coloring and the food dyes that you determined are found in that food coloring. Justify your choices by connecting the information presented in the table above to the data you collected for your gel. You should include a pic of your gel attached to this document as a reference. NOTE: it is possible that one or more of the dyes in the table above are not present in your sample.
Part 2: Bacterial transformation
Watch this video and answer the following questions: https://dnalc.cshl.edu/resources/dnatoday/2020-virtual-lab-bt.html
What gene of interest is being inserted into the bacteria? What organism is the original source of this gene? What trait does this gene convey?
List the steps that the instructor does and the reason/function for each step.
The plasmid used in this experiment has several important genes, as shown below:
GFP is the gene that conveys glowing, bla is the gene that conveys ampicillin resistance, and araC encodes for enzymes to metabolize a sugar called arabinose. GFP and araC share the same promoter, which is part of an inducible operon (similar to the lac operon).
What ingredient, not mentioned in the video, was necessarily present in the agar, in order for the bacteria to express GFP?
Explain how this ingredient specifically works in the operon.
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